Short Communication: Biochemistry Analysis and Molecular Approach to Identify the Cultured Bacterial from Ex-Tin Mining Lakes

Dr. Andri Kurniawan, S.Pi., M.P., - and Oedjijono, - and Tamad, - and Uyi Sulaeman, - (2022) Short Communication: Biochemistry Analysis and Molecular Approach to Identify the Cultured Bacterial from Ex-Tin Mining Lakes. Jurnal Ilmu Lingkungan, 20 (3). ISSN 1829-8907

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Abstract

There are two methods to identify the bacterial characteristic, namely biochemical analysis and the 16S ribosomal ribonucleic acid gene (16S rRNA) sequencing analysis. The research aimed to identify the cultured bacterial from ex-tin mining lakes by biochemistry analysis and molecular approach. Nine bacterial were cultured and isolated in nutrient agar and then biochemically characterized by microbact™ 12A and 24E (Oxoid) identification kits. In addition, molecular analysis by 16S rRNA gene was sequenced primer 1492R and primer 27F. Based on biochemistry analysis, these bacterial were identified as belonging to species of Bacillus amyloliquefaciens; Enterobacter gergoviae; Enterobacter aerogenes; Enterobacter agglomerans; and Nitrobacter spp. The sequence analysis in gene bank of NCBI indicated that these species had similarity with Klebsiella variicola strain F2R9 (Accession NR_025635.1); Enterobacter cloacae subsp. dissolvens strain LMG 2683 (Accession NR_044978.1); Serratia marcescens strain NBRC 102204 (Accession NR_114043.1); Bacillus marisflavi strain TF-11 (Accession NR_118437.1); Falsibacillus pallidus strain CW 7 (Accession NR_116287.1); Klebsiella pneumoniae strain DSM 30104 (Accession NR_117683.1); and Nitrobacter winogradskyi strain Nb-255 (Accession NR_074324.1). However, phylogenetic tree was constructed by Neighbor-Joining Test showed the cultured bacterial were not in the same clade and also with Salmonella enterica subsp. enterica strain LT2 (Accession NR_074910.1); Bacillus amyloliquefaciens strain BCRC 11601; and Escherichia coli strain NBRC 102203 (Accession NR_114042.1) as in group species and Micrococcus luteus strain NCTC 2665 (Accession NR_075062.2); Chloroflexus islandicus strain isl-2 (Accession NR_148571.2); Flavobacterium gondwanense (Accession M92278.1); and Cytophaga aurantiaca strain JM110 (Accession MN758870.1) as their out group.

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